Shallow resources are becoming increasingly depleted, deep resource exploration has become a global strategy. The design and testing of deep in-situ core samples are prerequisites for exploring deep resources; however, no in-situ condition-preserved coring and testing techniques and tools have been reported yet. Here, the first deep in-situ condition-preserved coring system (with the preservation of pressure, temperature, substance, light, and moisture) was developed that considers the effects of high water pressure and formation dynamic loads, along with an in-situ condition-preserved testing system. A pressure-preserved controller was designed, achieving the ultimate capacity of 140 MPa and 150 ℃. A temperature-preserved coring system combining active heating and passive insulation was constructed, realizing temperature preservation from room temperature to 150 ℃. Three generations of film-formation principles and methods were designed, achieving an excellent quality preserved rate, moisture preserved rate, and visible light barrier rate. Moreover, a deep in-situ condition-preserved coring system, and a simulated coring platform for large cores under in-situ environments was fabricated. A non-contact testing system was derived to cut and prepare specimens under in-situ environment and to perform non-contact non-destructive testing and true triaxial testing. The research findings can be successfully applied to deep coal and gas development, deep oil and gas resources assessment, and deep-sea sediment prospecting, achieving excellent application outcomes. This study provides important theoretical, technical and hardware support for deep in-situ rock physics and mechanics research and deep resource exploitation.