Although quantum-dot light-emitting diodes (QLEDs) can exhibit high efficiency and long lifetime, the realization of QLEDs-based displays remains challenging due to their complex multilayer architectures and the use of unstable PEDOT:PSS hole injection layer (HIL). Here, we develop a novel trilayer PIN QLED with only three functional layers: PTAA:TFB:F4-TCNQ bulk-heterojunction (BHJ) hole transport layer (HTL), quantum-dot emitting layer and ZnMgO electron transport layer. Due to well-matched energy level, increased hole transport path from PTAA to TFB, improved hole density and enhanced hole mobility of the PTAA:TFB:F4-TCNQ BHJ HTL, the resultant trilayer PIN QLED exhibits a high external quantum efficiency (EQE) of 25.1% and an impressive peak brightness of 382,600 cd/m², which are significantly higher than those of control QLED. Moreover, the trilayer PIN QLED also shows a 1.94-fold longer operational lifetime than control QLED due to the improved device performance, reduced charge accumulation, and removal of unstable PEDOT:PSS. The developed trilayer PIN QLED, with fewer functional layers and better stability, could promote the practical application of QLED in displays and solid-state lighting.